Protein Purification Techniques

Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set)are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.

Author
Publisher OUP Oxford
Release Date
ISBN 0191565741
Pages 278 pages
Rating 4/5 (48 users)

More Books:

Protein Purification Techniques
Language: en
Pages: 278
Authors: Simon Roe
Categories: Science
Type: BOOK - Published: 2001-01-25 - Publisher: OUP Oxford

Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to eluci
Protein Purification Techniques
Language: en
Pages: 262
Authors: Simon Roe
Categories: Proteins
Type: BOOK - Published: 2006 - Publisher:

Protein Purification Applications
Language: en
Pages: 182
Authors: Simon Roe
Categories: Science
Type: BOOK - Published: 2001-01-25 - Publisher: OUP Oxford

Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to eluci
PROTEIN PURIFICATION TECHNIQUES 2E
Language: en
Pages:
Authors: ROE
Categories:
Type: BOOK - Published: - Publisher:

Protein Purification
Language: en
Pages: 532
Authors: Jan-Christer Janson
Categories: Science
Type: BOOK - Published: 2012-01-03 - Publisher: John Wiley & Sons

The authoritative guide on protein purification—now completely updated and revised Since the Second Edition of Protein Purification was published in 1998, the
Effects of Protein Purification Techniques on the Immune Response
Language: en
Pages: 101
Authors: Azza El Amir
Categories: Medical
Type: BOOK - Published: 2015-12-28 - Publisher: diplom.de

This study discusses a very important issue in immune responses against different human diseases. Different immunological methods are to be used to evaluate the
Protein Purification Protocols
Language: en
Pages: 484
Authors: Paul Cutler
Categories: Science
Type: BOOK - Published: 2008-02-02 - Publisher: Springer Science & Business Media

The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims
Guide to Protein Purification
Language: en
Pages: 894
Authors: Murray P. Deutscher
Categories: Science
Type: BOOK - Published: 1990 - Publisher: Gulf Professional Publishing

Guide to Protein Purification, designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that prov
Protein Analysis and Purification
Language: en
Pages: 520
Authors: Ian M. Rosenberg
Categories: Science
Type: BOOK - Published: 2013-12-01 - Publisher: Springer Science & Business Media

How one goes about analyzing proteins is a constantly evolving field that is no longer solely the domain of the protein biochemist. Investi gators from diverse
Protein Purification
Language: en
Pages: 329
Authors: Robert K. Scopes
Categories: Science
Type: BOOK - Published: 2013-04-17 - Publisher: Springer Science & Business Media

New textbooks at alllevels of chemistry appear with great regularity. Some fields like basic biochemistry, organic reaction mechanisms, and chemical thermodynam